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3845 f5  (R&D Systems)


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    R&D Systems 3845 f5
    3845 F5, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3845 f5/product/R&D Systems
    Average 93 stars, based on 22 article reviews
    3845 f5 - by Bioz Stars, 2026-02
    93/100 stars

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    Hemolysis induced by patient, father’s and normal serum in the presence of factor H-related protein 5. a Serum from the patient (pink) and father (green), both bearing the CFHR5 variant M514R, induced hemolysis of rabbit red blood cells, inhibited by the addition of physiological concentrations of recombinant human factor H related 5 (hFHR5). Control sera ( n = 6) did not exhibit a significant change in hemolysis when hFHR5 was added. ns, not significant. b Serum from the father bearing the CFHR5 variant M514R induced hemolysis that was reduced when hFHR5 was added at higher than physiological concentrations. Similar results were obtained using control sera ( n = 7). * P < 0.01

    Journal: Pediatric Nephrology (Berlin, Germany)

    Article Title: Complement dysregulation associated with a genetic variant in factor H-related protein 5 in atypical hemolytic uremic syndrome

    doi: 10.1007/s00467-023-06184-6

    Figure Lengend Snippet: Hemolysis induced by patient, father’s and normal serum in the presence of factor H-related protein 5. a Serum from the patient (pink) and father (green), both bearing the CFHR5 variant M514R, induced hemolysis of rabbit red blood cells, inhibited by the addition of physiological concentrations of recombinant human factor H related 5 (hFHR5). Control sera ( n = 6) did not exhibit a significant change in hemolysis when hFHR5 was added. ns, not significant. b Serum from the father bearing the CFHR5 variant M514R induced hemolysis that was reduced when hFHR5 was added at higher than physiological concentrations. Similar results were obtained using control sera ( n = 7). * P < 0.01

    Article Snippet: Recombinant hFHR5 was used as the standard (catalog number: 3845-F5, R&D Systems).

    Techniques: Variant Assay, Recombinant, Control

    Factor H-related protein 5 wild-type and mutant proteins in cell supernatants and lysates. Factor H-related protein 5 was assayed by immunoblotting in transfected cell supernatants and lysates containing the wild-type sequence and the mutant M514R variant. Wild-type supernatant exhibited a strong band corresponding to FHR5. The supernatant from cells transfected with the CFHR5 M514R variant exhibited a very weak band. Control supernatants from HEK cells that were not transfected did not exhibit a band corresponding to FHR5. The FHR5 band was stronger in the lysates of the cells containing the M514R mutant variant compared to the wild-type, as the mutant variant was minimally secreted. hFHR5, recombinant human factor H-related protein 5; WT, wild-type; M514R, transfected mutant variant

    Journal: Pediatric Nephrology (Berlin, Germany)

    Article Title: Complement dysregulation associated with a genetic variant in factor H-related protein 5 in atypical hemolytic uremic syndrome

    doi: 10.1007/s00467-023-06184-6

    Figure Lengend Snippet: Factor H-related protein 5 wild-type and mutant proteins in cell supernatants and lysates. Factor H-related protein 5 was assayed by immunoblotting in transfected cell supernatants and lysates containing the wild-type sequence and the mutant M514R variant. Wild-type supernatant exhibited a strong band corresponding to FHR5. The supernatant from cells transfected with the CFHR5 M514R variant exhibited a very weak band. Control supernatants from HEK cells that were not transfected did not exhibit a band corresponding to FHR5. The FHR5 band was stronger in the lysates of the cells containing the M514R mutant variant compared to the wild-type, as the mutant variant was minimally secreted. hFHR5, recombinant human factor H-related protein 5; WT, wild-type; M514R, transfected mutant variant

    Article Snippet: Recombinant hFHR5 was used as the standard (catalog number: 3845-F5, R&D Systems).

    Techniques: Mutagenesis, Western Blot, Transfection, Sequencing, Variant Assay, Control, Recombinant